£€0€ €0€ €0€ €‹·¡€0€ €2BYP¡€remark 3: Refinement.¡€)remark 3: Program : Refmac 5.2.0005¡€0remark 3: Authors : Murshudov,Vagin,Dodson¡€2remark 3: Refinement Target : Maximum Likelihood¡€$remark 3: Data Used In Refinement.¡€4remark 3: Resolution Range High (Angstroms) : 2.07¡€5remark 3: Resolution Range Low (Angstroms) : 20.00¡€4remark 3: Data Cutoff (Sigma(F)) : None¡€5remark 3: Completeness For Range (%) : 99.76¡€5remark 3: Number Of Reflections : 83709¡€+remark 3: Fit To Data Used In Refinement.¡€9remark 3: Cross-Validation Method : Throughout¡€5remark 3: Free R Value Test Set Selection : Random¡€6remark 3: R Value (Working + Test Set) : 0.17386¡€6remark 3: R Value (Working Set) : 0.17305¡€6remark 3: Free R Value : 0.21357¡€3remark 3: Free R Value Test Set Size (%) : 2.0¡€3remark 3: Free R Value Test Set Count : 1700¡€.remark 3: Fit In The Highest Resolution Bin.¡€4remark 3: Total Number Of Bins Used : 20¡€7remark 3: Bin Resolution Range High (A) : 2.067¡€7remark 3: Bin Resolution Range Low (A) : 2.120¡€6remark 3: Reflection In Bin (Working Set) : 5925¡€7remark 3: Bin R Value (Working Set) : 0.231¡€5remark 3: Bin Free R Value Set Count : 121¡€7remark 3: Bin Free R Value : 0.282¡€remark 200: Experiment Type : X-Ray Diffraction¡€1remark 200: Date Of Data Collection : Null¡€0remark 200: Temperature (Kelvin) : 100¡€0remark 200: Ph : 7.5¡€.remark 200: Number Of Crystals Used : 1¡€-remark 200: Synchrotron (YN) : Y¡€?remark 200: Radiation Source : Als Beamline 8.2.1¡€2remark 200: Beamline : 8.2.1¡€1remark 200: X-Ray Generator Model : Null¡€-remark 200: Monochromatic Or Laue (ML) : M¡€2remark 200: Wavelength Or Range (A) : 0.976¡€1remark 200: Monochromator : Null¡€1remark 200: Optics : Null¡€1remark 200: Detector Type : Null¡€1remark 200: Detector Manufacturer : Null¡€2remark 200: Intensity-Integration Software : Denzo¡€6remark 200: Data Scaling Software : Scalepack¡€2remark 200: Number Of Unique Reflections : 86601¡€1remark 200: Resolution Range High (A) : 2.07¡€2remark 200: Resolution Range Low (A) : 50.00¡€0remark 200: Rejection Criteria (Sigma(I)) : 0.0¡€remark 200: Overall.¡€1remark 200: Completeness For Range (%) : 99.9¡€0remark 200: Data Redundancy : 5.1¡€1remark 200: R Merge (I) : 0.08¡€1remark 200: R Sym (I) : Null¡€1remark 200: FOR THE DATA SET : 29.50¡€,remark 200: In The Highest Resolution Shell.¡€;remark 200: Highest Resolution Shell, Range High (A) : Null¡€;remark 200: Highest Resolution Shell, Range Low (A) : Null¡€1remark 200: Completeness For Shell (%) : Null¡€1remark 200: Data Redundancy In Shell : Null¡€1remark 200: R Merge For Shell (I) : Null¡€1remark 200: R Sym For Shell (I) : Null¡€0remark 200: FOR SHELL : NULL¡€3remark 200: Diffraction Protocol: Single Wavelength¡€Iremark 200: Method Used To Determine The Structure: Molecular Replacement¡€ remark 200: Software Used: Amore¡€*remark 200: Starting Model: Pdb Entry 2byn¡€remark 200: Remark: None¡€remark 280: Crystal¡€+remark 280: Solvent Content, Vs (%): 49.25¡€9remark 280: Matthews Coefficient, Vm (Angstroms3DA): 2.44¡€Gremark 280: Crystallization Conditions: 11-14% Peg-4000, 0.1 M Tris, Ph¡€remark 280: 7.5, 0.4 M Mgcl2¡€%remark 290: Crystallographic Symmetry¡€7remark 290: Symmetry Operators For Space Group: I 2 2 2¡€remark 290: Symop Symmetry¡€remark 290: Nnnmmm Operator¡€remark 290: 1555 X,Y,Z¡€remark 290: 2555 -X,-Y,Z¡€remark 290: 3555 X,-Y,-Z¡€remark 290: 4555 -X,Y,-Z¡€!remark 290: 5555 X+12,Y+12,Z+12¡€#remark 290: 6555 -X+12,-Y+12,Z+12¡€#remark 290: 7555 X+12,-Y+12,-Z+12¡€#remark 290: 8555 -X+12,Y+12,-Z+12¡€(remark 290: Where Nnn -> Operator Number¡€%remark 290: Mmm -> Translation Vector¡€5remark 290: Crystallographic Symmetry Transformations¡€Cremark 290: The Following Transformations Operate On The AtomHETATM¡€Aremark 290: Records In This Entry To Produce Crystallographically¡€remark 290: Related Molecules.¡€Cremark 290: Smtry1 1 1.000000 0.000000 0.000000 0.00000¡€Cremark 290: Smtry2 1 0.000000 1.000000 0.000000 0.00000¡€Cremark 290: Smtry3 1 0.000000 0.000000 1.000000 0.00000¡€Cremark 290: Smtry1 2 -1.000000 0.000000 0.000000 0.00000¡€Cremark 290: Smtry2 2 0.000000 -1.000000 0.000000 0.00000¡€Cremark 290: Smtry3 2 0.000000 0.000000 1.000000 0.00000¡€Cremark 290: Smtry1 3 1.000000 0.000000 0.000000 0.00000¡€Cremark 290: Smtry2 3 0.000000 -1.000000 0.000000 0.00000¡€Cremark 290: Smtry3 3 0.000000 0.000000 -1.000000 0.00000¡€Cremark 290: Smtry1 4 -1.000000 0.000000 0.000000 0.00000¡€Cremark 290: Smtry2 4 0.000000 1.000000 0.000000 0.00000¡€Cremark 290: Smtry3 4 0.000000 0.000000 -1.000000 0.00000¡€Cremark 290: Smtry1 5 1.000000 0.000000 0.000000 65.14200¡€Cremark 290: Smtry2 5 0.000000 1.000000 0.000000 70.01800¡€Cremark 290: Smtry3 5 0.000000 0.000000 1.000000 76.69300¡€Cremark 290: Smtry1 6 -1.000000 0.000000 0.000000 65.14200¡€Cremark 290: Smtry2 6 0.000000 -1.000000 0.000000 70.01800¡€Cremark 290: Smtry3 6 0.000000 0.000000 1.000000 76.69300¡€Cremark 290: Smtry1 7 1.000000 0.000000 0.000000 65.14200¡€Cremark 290: Smtry2 7 0.000000 -1.000000 0.000000 70.01800¡€Cremark 290: Smtry3 7 0.000000 0.000000 -1.000000 76.69300¡€Cremark 290: Smtry1 8 -1.000000 0.000000 0.000000 65.14200¡€Cremark 290: Smtry2 8 0.000000 1.000000 0.000000 70.01800¡€Cremark 290: Smtry3 8 0.000000 0.000000 -1.000000 76.69300¡€remark 290: Remark: Null¡€remark 300: Biomolecule: 1¡€Dremark 300: This Entry Contains The Crystallographic Asymmetric Unit¡€>remark 300: Which Consists Of 10 Chain(S). See Remark 350 For¡€Aremark 300: Information On Generating The Biological Molecule(S).¡€:remark 300: Quaternary Structure For This Entry: Decameric¡€?remark 300: The Hetero-Assembly Described By Remark 350 Appears¡€7remark 300: To Be A Case Of Strong Crystal Packing With¡€>remark 300: The Mean Difference In Accessible Surface Area Per¡€9remark 300: Chain Between The Isolated Chain And That For¡€¡€ 56 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€0€ €?¡€ 57 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€0€ €@¡€ 58 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€0€ €A¡€ 59 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€0€ €B¡€ 60 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€40€ €C¡€ 61 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€"0€ €D¡€ 62 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€0€ €E¡€ 63 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€0€ €F¡€ 64 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€.0€ €G¡€ 65 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€0€ €H¡€ 66 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dictionary¡€ €¡€70€ €œ¡€ 150 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€.0€ €¡€ 151 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€0€ €ž¡€ 152 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€(0€ €Ÿ¡€ 153 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€0€ € ¡€ 154 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€0€ €¡¡€ 155 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€ 0€ €¢¡€ 156 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€0€ €£¡€ 157 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€"0€ €¤¡€ 158 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€10€ €¥¡€ 159 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€ 0€ €¦¡€ 160 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€10€ €§¡€ 161 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€ 0€ €¨¡€ 162 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€0€ €©¡€ 163 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€:0€ €ª¡€ 164 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€ 0€ €«¡€ 165 ¢€¢€0€ €¡€0€ €Standard residue dictionary¡€ €¡€0€ €¬¡€ 166 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