VIVA LA JOSEPH (LIVING FOR JOSEPH)


Taylor, Casey, Evan, Dom, Emilee, Raven

Intro

Our stay at GTAC was extraordinary. We were able to use facilities and equipment that would not be able to access in rural Victoria. We also gained the opportunity to meet many professionals who currently work in the science fields, one of which was Joseph who aided us in our wiggle assay experiment. Joseph inspired us so much that we thought we should honour him by naming our group ‘Viva La Joseph’.

Wiggle Assay

Figure 1. The wells we used for the Wriggle Assay

The data we collected from the original fruit fly (Drosophila Melanogaster) shows that it is susceptible to all three insecticides. The data for the mutant Dα6KO (D Alpha Six Knock Out) shows us that the mutant Dα6KO is immune to the insecticide Spinosad. Dα6KO is susceptible to the insecticides Chorantranilliprole and Imidacloprid, the mutant Dα1KO (D Alpha Six Knock Out) was immune to the insecticide Imidacloprid but was susceptible to the insecticides Chlorantranilliprole. The insecticide Chlorantranilliprole is the only insecticide that killed all three fruit fly.

The data suggests that if one was to use a mixture of two or more insecticides it would have a better chance of exterminating the fruit fly.

Electron Microscope

The electron microscope was a great experience for us, we never knew it was possible to zoom up on such a small object over 18,000 times! We looked at the wild type fly and the tm3-tm6 (mutant). The mutant had a change in its DNA forcing it to grow with curled wings. It looked odd but incredible at the same time.

Figure 2. An image of a fly's eye under an electron microscope

 

 

 

 

 

 

 

 

Our experience

  • Taylor: “During my time at GTAC I found that I really enjoyed using the equipment in the labs such as the micropipette and the electron microscope, my favourite part of the experience was the gel electrophoresis and extracting the DNA.”
  • Casey: “I found that the modern facilities and equipment we used were really interesting.”
  • Evan: “I enjoyed using the electron microscope as it showed a different image to a light microscope.”
  • Dom: “The overall experience at GTAC was amazing and it broadened my knowledge of the world of biology.”
  • Emilee: “I enjoyed learning about DNA and how we are able to modify pieces individually.”
  • Raven:” I enjoyed my time at GTAC because I learnt a lot and used equipment and devices that I would not be able to use at school. The things I learnt at GTAC are taught to year 10’s,11’s and 12’s and as a year 9 the knowledge that I have gained will help me and give me a head start in the years to come.”

Photo Gallery

 

Figure 3. A gel electrophoresis machine

 

 

 

 

 

 

 

 

 

 

 

 

Figure 4. Drosophila larvae

 

 

 

 

 

 

 

 

Figure 5. Preparing the wells for the Wiggle Assay